Roche rolls out new DNA ‘sequencing-by-expansion’ approach

Roche has unveiled a revolutionary new DNA sequencing technology called Sequencing by Expansion (SBX). Here are the key details about this innovative approach:

Technology Overview

SBX uses a novel biochemical process to encode DNA sequence information into an expanded surrogate molecule called an Xpandomer12. Key features include:

• Expands DNA to 50 times its original length, allowing for clearer signal detection13
• Uses modified nucleotides called X-NTPs to create the Xpandomer24
• Combines aspects of DNA synthesis and nanopore-based molecule reading1

How It Works

1. X-NTPs are sequentially incorporated along a DNA template4
2. The DNA template is then degraded and the X-NTP backbone expands2
3. The resulting Xpandomer is fed through nanopores on a CMOS-based sensor4
4. Changes in electrical voltage correspond to each DNA base sequence1

Advantages

- Improved Signal-to-Noise Ratio:
The expanded molecule provides clearer signals for more accurate sequencing28

- Speed:
Capable of sequencing hundreds of millions of bases per second48

- Flexibility:
Can handle both small and large-scale sequencing projects on the same system18

- Scalability:
Uses parallel processing across millions of nanopores48

Applications and Availability

• Suitable for whole genome sequencing, exome sequencing, and RNA sequencing8
• Potential applications in both research and clinical settings12
• Currently in development, with plans for commercial launch in 202612

This technology represents a significant breakthrough in DNA sequencing, potentially revolutionizing genomic research and healthcare applications8.

Sources:

1. https://www.fiercebiotech.com/medtech/roche-rolls-out-new-dna-sequencing-expansion-approach

2. https://www.genengnews.com/topics/omics/roche-announces-sbx-technology-creates-sequencing-buzz/

3. https://www.noahai.co/discover/article/3322

4. https://www.youtube.com/watch?v=G8ECt04qPos

8. https://www.roche.com/media/releases/med-cor-2025-02-20