Simplifying Manufacturing of Alphavirus Self-Amplifying RNA Replicons ###

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1. Alphavirus Self-Amplifying RNA Replicons: These replicons are increasingly popular for vaccine development, offering the benefits of both live attenuated and subunit vaccines.
2. Manufacturing Process: The process is cell-free, rapid, and highly scalable, allowing for rapid deployment to novel disease targets.
3. CleanCap Co-Transcriptional Capping: This method simplifies the capping step, providing high capping efficiency and yield, and reducing manufacturing times.
4. In Vitro Transcription: Replicons are made by in vitro transcription, which allows for rapid and scalable production.
5. Advantages: Self-amplifying RNA replicons require very low doses, are amenable to repeat dosing, and stimulate innate immunity, enhancing vaccination.